dextran hydrodynamic radius

Kirkland, J. J.; Dilks, C. H.; Rementer, S. W. (1992). Ibrahim O, Munavalli GS, Dover JS. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol The skin poration was performed using the microporation device (LG electronics, Korea). After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. Drug Discov Today Technol. Chem. The authors declare that they have no competing interests. Bethesda, MD 20894, Web Policies TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. 2013;43(4):287303. 43-1, 115-147. Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. Front Microbiol. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. TEWL values before and immediately after microporation on human cadaver skin according to the number of times microproration was done (n=3). WebR H Hydrodynamic radius [m] The basic DLS setup. The enhanced permeability through micropores was confirmed by Franz diffusion cell. Carbohydrate Research, 160, 205-214. WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). sharing sensitive information, make sure youre on a federal The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. All authors have consented to the submission of this manuscript for publication. Macromolecules, 33, 5730-5739. 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0. Dextran. Cite this article. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. (2003). TEWL measurements were taken 30min after microporation of the human and pig skin. WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. Prausnitz MR, Langer R. Transdermal drug delivery. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which WebDextran produced from Weissella cibaria JAG8 was purified and characterized. De Belder, A. N. (2001). Food Sci Biotechnol. Biomaterials, 22, 1891-1898. Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect. 2011;5(2):95132. At 25C both values of a and kM-H are within the range reported by [3, 8, 16]. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol 2004;25(9):155967. 2014 Sep;5(9):2324-30. doi: 10.1039/c4fo00319e. Dextran Utilization During Its Synthesis by Weissella cibaria RBA12 Can Be Overcome by Fed-Batch Fermentation in a Bioreactor. The exponent a is a function of polymer geometry, and varies from 0.5 to 2 and exponent is dimensionless. 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. Journal Non-Newtonian Fluid Mech., 97, 295-301. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. This conjecture found to be correct. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Carbohydrate Polymers, 79, 380-390. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. SWC designed and coordinated the research. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. The Mark-Houwink a constant is close to 0.5 in poor solvents. In fact, short chains do contain minor branching points. 2005;22(4):5505. By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. 2. Alternatives to animal testing: a review. For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. Carbohydrate Polymers, 36, 319-326. Molecular weight determination of polysaccharides. 113, 81-86. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. Keywords: FITC dextran; intestinal permeability; transit time. Formul. The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). Ann Surg Oncol. FITC-dextran solution (600L) was added to the donor chamber. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. Drug Deliv. Clipboard, Search History, and several other advanced features are temporarily unavailable. Advanced Drug Delivery Reviews, 60, 1650-1662. Dextrans in Aqueous Solution. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows Biomater Res 24, 22 (2020). Biomaterials Research Viscosity of Liquids. Ahn, G.Y., Eo, HS., Kim, D. et al. ; Veldhuis, T.F.J. Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. Chem. Increased epidermal laser fluence through simultaneous ultrasonic microporation. In order to confirm the molecular weight the gel permeation chromatography (GPC) for standard dextrans is used in a range of molecular weights from 8.8 to 2000kDa and these correspond to the following equation. PMC Before In the case of the work performed in references [34, 35, 36] they studied using the number average molecular weight, which rearranges the value of a and forces it to enter into unbranched or linear molecules, on the other hand, if molecular weights are used the value would be less than 0.5. (2003). Doke SK, Dhawale SC. 20K groups (n=3, *: p<0.05, **: p<0.01). The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly 1905/05) for their financial support. In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). ; Fran, H.P. Soc., 21, 1, 78-86. J. Phys. Recent Pat Drug Deliv Formul. Calculation of MarkHouwinkSakurada (MHS) equation viscometric constants for chitosan in any solventtemperatura system using experimental reported viscometric constants data. Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC All branched polysaccharide types could be described approximately by the hyperbranching theory. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. 2015;32(5):170413. Proc. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation. Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. Epub 2022 Feb 2. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. Careers. statement and ; van Nostrum, C.F. Figure4 shows the histological analysis of micropores in human cadaver skin. Google Scholar. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. The https:// ensures that you are connecting to the Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. 2019;9(3-S):9726. Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. (2008). 2008;26(11):12618. SEC of mono-carboxymethyl cellulose (CMC) in a wide range of pH, MarkHouwink constants. Polyelectrolytes as secondary calibration standards for aqueous SEC. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. Appl Biochem Biotechnol. Macromolecules, 29, 2265-2268. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. Journal of American Chemical Society, 64(11), 2716-2718. Correspondence to Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. The radius of gyration is typically calculated from static scattering measurements, while the hydro-dynamic radius (or equivalent sphere radius) is deter-mined by dynamic light scattering or other diffusion measurements. Table 1 shows some values of dextrans Mark-Houwink parameters, from literature consulted, where all data are based on the logarithmic plot of intrinsic viscosity [] and molecular weight Mw. Guner, Ali (1999). Webwhittier union high school district superintendent. Food Funct. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. For a values from 0-0.5 a rigid sphere is predicted in a ideal solvent, from 0.5-0.8 a random coil in good solvent, and from 0.8-2 a rigid or rod like shape is expected (stiff chain). ; Yevlampieva N.P. Biomacromolecules 8, 2464-2475. Microporation was performed 2, 5, and 10 times on each sample. The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). WebDextran 40 Pharmaceutical Quality. 4K, b M.W. Viscotek GmbH, Europe. Journal of Applied Polymer Science, 13, 2031-2032. This situation is mainly the result of using dextans, solvent, additives, etc. Pure Appl. On the molecular weight distribution of dextran T-500. The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. Ind. Gillespie, T.; Hulme, M. A. The hydrodynamic radius of 2-NBDG glucose was too small to measure. Working with the molecular weights given by the manufacturer and compare them with the molecular weights, calculated from the Mark-Houwink parameters in this work; can be seen that the %RE obtained, see Table 6. The receptor chamber was filled with PBS. Our portfolio includes for example derivatives of dextran, polysucrose, inulin and more. Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. Chem. Harding, S. E., & Simpkin, N. J. 2010;4(1):117. 1994, 95-0355. European Polymer Journal, 42, 1086-1092. The solution was measured using an Anton Paar DMA35N densitometer. Guner, A.; Kibarer, G. (2001). Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. ; Weisz, J. On single point determination of intrinsic viscosity. Environ Toxicol Pharmacol. Learn More. A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). In addition, the permeation rate was higher in pig skin than in human skin. 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. Google Scholar. This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. 2012;29(7):201729. Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. Carbohydrate Polymers, 39, 377-380. High Manufacturing Standards of Molecular Probes Dextrans.

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